What is a gamma dog?

Canine IFN gamma (Yeast-derived Recombinant Protein) — 25 micrograms

Interferon gamma ( IFNγ ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Alternate Names — IFNG, IFG, IFI, interferon, gamma, interferon gamma

IFNγ Homology Across Species
Canis lupus familiaris (dog) IFNγ – 100%
Canis lupus dingo (dingo) IFNγ – 100%
Vulpes vulpes (red fox) IFNγ – 100%
Nyctereutes procyonoides (raccoon dog) IFNγ – 100%
Phoca vitulina (harbor seal) IFNγ – 100%
Ailurus fulgens (lesser panda) IFNγ – 96%
More — https://blast.ncbi.nlm.nih.gov

• Product Information
• Citations
• Ordering Information

Catalog No.:
RP0271D-025
Quantity:
25 ug
Source:

The Canine IFN gamma recombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.

The Canine IFN gamma recombinant protein has a predicted molecular weight of 16.7 kDa.
Protein Sequence:

QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK (143)

Country of Origin:
Applications:

The Canine IFN gamma endotoxin-free recombinant protein can be used in cell culture, as an IFN gamma ELISA Standard, and as a Western Blot Control.

TNF-α and INF-γ primed canine stem cell-derived extracellular vesicles alleviate experimental murine colitis

An JH, Li Q, Bhang DH, Song WJ, Youn HY.

Sci Rep. 2020 Feb 7;10(1):2115. doi: 10.1038/s41598-020-58909-4.

Applications: Stimulation of stem cells

Abstract

The inflammatory bowel diseases (IBD) are characterized by relapsing inflammation and immune activation diseases of the gastrointestinal tract. Extracellular vesicles, which elicit similar biological activity to the stem cell themselves, have been used experimentally to treat dextran sulfate sodium (DSS)-induced colitis in murine models though immunosuppressive potential. In this study, we investigated whether the Extracellular vesicles (EVs) obtained by stimulating inflammatory cytokine on canine adipose mesenchymal stem cells (cASC) improved anti-inflammatory and/or immunosuppressive potential of EVs, and/or their ability to alleviate inflammation in colitis. We also explored the correlation between immune cells and the inflammatory repressive effect of primed EVs. Pro-inflammatory cytokines such as TNF -α and IFN-γ increased immunosuppressive protein such as HGF, TSG-6, PGE2 and TGF-β in EVs. Moreover, the anti-inflammatory effect of EVs was improved through pretreatment with inflammatory cytokines. Importantly, EVs obtained from primed stem cells effectively induced macrophage polarization toward an anti-inflammatory M2 phenotype and suppressed activated immunity by enhancing regulatory T cells in inflamed colon in mice. Our results provide a new and effective therapy for the EVs obtained from ASC stimulated with TNF -α and IFN-γ against not only IBD, but also immune-mediated disease.

Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells.

Osterbur K, Yu DH, Declue AE.

Res Vet Sci. 2012 Nov 8. doi:pii: S0034-5288(12)00305-0. 10.1016/j.rvsc.2012.10.002.

Applications: Stimulation of canine aortic endothelial cells

Abstract

The N-terminal portion of pro C-type natriuretic peptide (NT-pCNP) has shown promise as a biomarker for sepsis in humans and dogs, however the mechanism of NT-pCNP production in dogs is unknown. Canine aortic endothelial cells were stimulated with lipopolysaccharide, lipoteichoic acid, peptidoglycan, TNF-α, IL-1β, IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A or control (PBS), and NT-pCNP production was measured. Lipopolysaccharide, TNF-α, and IL-1β significantly stimulated NT-pCNP production in a dose and time dependent manner; IL-1β resulted in the greatest NT-pCNP concentrations. The other stimulants did not result in significant NT-pCNP production. The addition of serum to the cell culture model did not alter lipopolysaccharide, lipoteichoic acid or peptidoglycan induced NT-pCNP production. These data indicate that lipopolysaccharide, TNF-α and IL-1β regulate CNP production from canine vascular endothelium and of the stimulants tested, IL-1β is the predominant inducing factor. These data provide some initial insight into the mechanisms of CNP regulation in dogs.

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Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114
651-646-0089
Fax: 651-646-0095
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IFN Gamma

Datasheet Datasheet

Product Description

Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.

Product Details

Target Species Dog
Product Form Purified recombinant protein — lyophilized

Reconstitution Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography

Source Pichia pastoris
Buffer Solution Phosphate buffered saline
Preservative Stabilisers 10% Trehalose
Purity >95% by SDS PAGE analysis
Protein Molecular Weight 16.7 kDa
Regulatory For research purposes only
Guarantee 12 months from date of despatch

Storage Information

Prior to reconstitution store at -20 o C.
After reconstitution store at -20 o C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

Applications

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2977 as a coating antibody and AHP2977B as a detection antibody.

Bioinformatics

Synonyms IFNG UniProt P51742 Entrez Gene TNF GO Terms GO:0005125 cytokine activity GO:0005615 extracellular space

Batch/Lot Specific Datasheets

PDP010

144592 159158 159697 161383

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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